| TSH

Thyroid Stimulating Hormone (TSH) is a pituitary hormone that stimulates the thyroid gland to produce thyroxine (T4), and then triiodothyronine (T3) which stimulates the metabolism of almost every tissue in the body. TSH is a glycoprotein and consists of two subunits, the alpha and the beta subunit. It is a glycoprotein hormone produced by thyrotrope cells in the anterior pituitary gland, which regulates the endocrine function of the thyroid.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	500	250	125	62.5	31.2	15.6	7.8	0
OD	2.36	1.49	0.95	0.54	0.35	0.26	0.21	0.09
Corrected OD	2.27	1.4	0.86	0.45	0.26	0.17	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human TSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	95

 

| Linearity

Samples were spiked with high concentrations of Human TSH and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)
1 : 2	Range (%)	84-95
	Average (%)	91
1 : 4	Range (%)	89-102
	Average (%)	95